Endocrine Abstracts

Objective: Ovarian cryopreservation is an emerging technique that may advance breeding of endangered species and preservation of reproductive capacity in young women cancer patients who will undergo chemo/radiotherapy destruction of ovarian tissue.Materials and methods: A total of twelve mature female rabbits were used in experiment. Rabbits were anesthetized and then subjected to laparotomy. Bilateral oophorectomy was used to save one ovary as fresh con...

A 31 year old man with a 12 month history of uncontrolled hypertension was referred for further investigation. He complained of easy bruising, muscle weakness and severe back and hip pains. Clinical signs included cushingnoid facies, centripetal obesity, atrophic skin, peripheral oedema and BP of 150/104. Initial investigations confirmed Cushing's syndrome with 24 hour urinary free cortisol 696 nanomols per litre and 9am plasma cortisol 658 nanomols per litre following midnigh...

Introduction: We have generated a truncated Growth Hormone Receptor (GHR) fused to the cell membrane by a glycosyl phosphatidylinositol anchor (GHR-GPI). The protein consists of the complete extracellular domain of the GHR, but lacks the intracellular domain, and we predict it would act as a GH antagonist. Aim: To test if partially-purified soluble GHR-GPI could re-insert into CHO cell membranes. Material and methods: Membrane preparations were made from CHO cells stably expre...